August 2022 Production of human A 2A AR in lipid nanodiscs for 19 F-NMR and single-molecule fluorescence spectroscopy "We describe production of the human A2A adenosine receptor (A2AAR), a class A G protein-coupled receptor (GPCR) for 19F-NMR and single-molecule fluorescence (SMF) spectroscopy. A2AAR and assembly of A2AAR in lipid nanodiscs and procedures for incorporation of either 19F-NMR or fluorescence

October 2022 Identification of A2BAR as a potential target in colorectal cancer using novel fluorescent A2BAR), specifically expressed in cancer cell lines compared with stromal cells, to explore the use of fluorescent Fluorescent probes allowed semi-quantitative receptor mapping in living cells and validated the specific As well, fluorescent ligands were effective at monitoring real-time A2BAR receptor labeling using live-imaging This proof-of-concept study suggests the use of fluorescent ligands for GPCR characterization through

We first synthesized a fluorescent ligand enabling equilibrium and kinetic binding studies via NanoBRET as well as fluorescence microscopy.

Using a bimolecular fluorescence complementation (BiFC) assay, we identified the β2 adrenergic receptor To investigate the molecular scale details of CXCR4-β2AR interactions, we used a time-resolved fluorescence spectroscopy method called pulsed-interleaved excitation fluorescence cross-correlation spectroscopy , Jae-Yeon Jeong , Won-Ki Huh , Niña G Caculitan , Adam W Smith Tags G-protein-coupled receptors , fluorescence spectroscopy , membrane protein interactions Source Contribute to the GPCR News Coming soon Become a

techniques including various mass spectrometry approaches, single particle cryo-EM and single-molecule fluorescence spectroscopy.

We have developed an array of fluorescent biosensors to map kinase activity in living cells and are exploring