August 2022 "The atypical chemokine receptor 1 (ACKR1) was discovered on erythrocytes as the Duffy blood group antigen ( Cutbush et al., 1950 ), also called Duffy-antigen/receptor for chemokines, or DARC ( Novitzky-Basso and Rot, 2012 ). Erythrocytes are terminally differentiated anuclear cells with no transcription and limited translation. Accordingly, within the erythroid lineage ACKR1 expression occurs first and is the highest in erythroblasts ( Duchene et al., 2017 ). Additionally, ACKR1 expression characterizes venular endothelial cells (ECs) ( Pruenster et al., 2009 ; Thiriot et al., 2017 ), including those lining bone marrow (BM) sinusoids ( Duchene et al., 2017 ). This well-established, distinctive pattern of cell expression has been directly challenged by a publication purporting ACKR1 expression in mouse BM by macrophages, but not erythroblasts and ECs, suggesting that macrophage ACKR1 engages its non-cognate ligand CD82 on hematopoietic stem cells (HSCs) to maintain their quiescence ( Hur et al., 2016 ). In light of the extensive literature, these findings have been particularly provocative, as this was the first description of ACKR1 expression by any leukocyte type and, if correct, would change current concepts of ACKR1 involvement in pathophysiology. The reported ACKR1 expression by macrophages in Hur et al. relied on using commercial anti-ACKR1 antibody FAB6695, which has neither been validated by the manufacturer nor by the authors. This prompted us to investigate the specificity of FAB6695 and scrutinize the apparent ACKR1 expression in BM macrophages" Read more at the source #DrGPCR #GPCR #IndustryNews

migration assay, performed on macrophages derived from both the THP-1 cell line and human peripheral blood monocytes

activation, chemotaxis and recruitment of multiple immunological cells such as microglia, macrophages and monocytes

< GPCR News < GPCRs in Oncology and Immunology CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes Published date March 21, 2023 Abstract "Dendritic cells (DCs) and monocytes Since only migratory DCs express this chemokine receptor, it is unclear how monocytes reach the LN. Migratory monocytes expressed Ccr5, a high-affinity receptor for Ccl5. Ccr7-, and Batf3-deficient mice demonstrated that while antigen-bearing DCs use CCR7 to reach the LN, monocytes

US28 by incoming HCMV particles rapidly attenuates Akt activity to suppress HCMV lytic replication in monocytes Published date August 27, 2024 Abstract "Establishing a nonproductive, quiescent infection within monocytes We investigated the mechanisms through which HCMV establishes a quiescent infection in monocytes. We found that preformed US28 was rapidly delivered to monocytes by HCMV viral particles, whereas the activity by limiting HCMV-induced EGFR activation during viral entry, enabling quiescent infection in monocytes

method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes , and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well