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August 2022 "The atypical chemokine receptor 1 (ACKR1) was discovered on erythrocytes as the Duffy blood group antigen ( Cutbush et al., 1950 ), also called Duffy-antigen/receptor for chemokines, or DARC ( Novitzky-Basso and Rot, 2012 ). Erythrocytes are terminally differentiated anuclear cells with no transcription and limited translation. Accordingly, within the erythroid lineage ACKR1 expression occurs first and is the highest in erythroblasts ( Duchene et al., 2017 ). Additionally, ACKR1 expression characterizes venular endothelial cells (ECs) ( Pruenster et al., 2009 ; Thiriot et al., 2017 ), including those lining bone marrow (BM) sinusoids ( Duchene et al., 2017 ). This well-established, distinctive pattern of cell expression has been directly challenged by a publication purporting ACKR1 expression in mouse BM by macrophages, but not erythroblasts and ECs, suggesting that macrophage ACKR1 engages its non-cognate ligand CD82 on hematopoietic stem cells (HSCs) to maintain their quiescence ( Hur et al., 2016 ). In light of the extensive literature, these findings have been particularly provocative, as this was the first description of ACKR1 expression by any leukocyte type and, if correct, would change current concepts of ACKR1 involvement in pathophysiology. The reported ACKR1 expression by macrophages in Hur et al. relied on using commercial anti-ACKR1 antibody FAB6695, which has neither been validated by the manufacturer nor by the authors. This prompted us to investigate the specificity of FAB6695 and scrutinize the apparent ACKR1 expression in BM macrophages" Read more at the source #DrGPCR #GPCR #IndustryNews

For instance, the expression of specific chemokine receptors in monocytes and macrophages indicates their specific contributions.Moreover, the study's findings on the unique expression of chemokine receptors in monocytes

migration assay, performed on macrophages derived from both the THP-1 cell line and human peripheral blood monocytes

activation, chemotaxis and recruitment of multiple immunological cells such as microglia, macrophages and monocytes

primarily expressed in myeloid cells that constitute the innate immune system, including neutrophils, monocytes

Since many inflammatory diseases are driven by inappropriate recruitment of monocytes into tissues, CCR2 In this model, OB-004 showed powerful efficacy, achieving full blockade of monocyte recruitment in response Figure 5: In an in vitro functional signaling assay on a human monocytic cell line, OB-004 demonstrated Figure 6: In a flow-based human monocyte endothelial transmigration assay, OB-004 strongly outperformed Twice-daily treatment with mOB-004 dose-dependently suppressed monocyte infiltration into the peritoneum

Interestingly, in monocytes and dendritic cells exposed to treatments mimicking inflammation, CCR1, CCR2

affect constitutive receptor internalization, with the same observations made for CCR2-expressing THP-1 monocytic In monocytes and dendritic cells exposed to treatments mimicking inflammation, CCR1, CCR2, and CCR5 switch

CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes.

October 2022 Dual loss of regulator of G protein signaling 2 and 5 exacerbates ventricular myocyte arrhythmias

US28 by incoming HCMV particles rapidly attenuates Akt activity to suppress HCMV lytic replication in monocytes

Updates in GPCR Research Transcriptome analysis of Schizothorax oconnori (Cypriniformes: Cyprinidae) oocytes : The role of K+ in promoting yolk globule fusion and regulating oocyte maturation 19F-NMR studies of

Ligands GPCRs in Cardiology, Endocrinology, and Taste Mechanistic Insights Into Redox Damage of the Podocyte

neuronal GPCR shapes behavior Methods & Updates in GPCR Research Advancements in the use of xenopus oocytes