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My project involved the development of FRET/BRET -based GPCR conformational biosensors, which can be

optical biosensors based on fluorescence or bioluminescence resonance energy transfer technologies (FRET , BRET) that were used for functional screens with state-of-the-art microscopy and high throughput screening

Research director at INSERM with a specific interest in G protein-coupled receptors by developing original BRET and TR-FRET assays.

Bouvier on BRET, pharmacological chaperones, biased agonism, and why unexpected data is where real GPCR discovery begins. << Back to podcast list Strategic Partner(s) Michel Bouvier: BRET, Biased Agonism, From the development of BRET-based biosensors that revealed receptor behavior in living cells, to the It describes the actual origin of BRET, pharmacological chaperones, and the inverse agonism work - each BRET was built in response to a rejection The technology that would become BRET emerged from a peer-review

trials weren't ready, what it takes to hold a GPCR signaling lab together during a pandemic, and the BRET-based receptor pharmacology work already in the pipeline before the shutdown, including a newly accepted BRET-based His research spans BRET-based assay platforms for characterizing signaling downstream of specific GPCRs BRET platforms carry work forward when the lab can't. The beta-1 adrenergic receptor paper — a BRET-based platform for capturing downstream signaling, built

formation, addiction, autism, schizophrenia, bipolar disorder, and tumorigenesis, applying cell biology, BRET and FRET assays, microscopy, flow cytometry, and custom protein engineering approaches to probe adhesion Choosing Mexico City as a Scientific Bet After postdoctoral and faculty positions at Stanford and UT formation, addiction, autism, schizophrenia, and cancer: the lab's research logic 01:13:45 Assays: BRET , FRET, microscopy, flow cytometry, and engineering membrane-anchored ligands into solution 01:19:29

biological, not chemical Polypharmacology for complex neuropsychiatric disease Functional assay design — BRET The bet is that biased ligands at the serotonin 2A receptor can separate the two. BRET captures the initial event; second messengers can mislead you. Roth's lab leans heavily on BRET-based G protein and arrestin assays because they're relatively insensitive psychedelic for depression 38:21 "I am not uncertain" — the data Bryan won't talk about yet 47:13 Why BRET

His group developed BRET and NanoBRET-based live-cell platforms that capture protein-protein interactions He developed BRET and NanoBRET-based live-cell assay platforms now widely adopted across the GPCR field BRET Went From a Handful of Labs to the Go-To Platform When Pfleger began working with bioluminescence He also has globally-recognized expertise in bioluminescence resonance energy transfer (BRET) technology

structure/function relationships of GPCRs using live-cell bioluminescence resonance energy transfer (BRET

structure/function relationships of GPCRs using live-cell bioluminescence resonance energy transfer (BRET

As part of Bouvier’s team, Bianca used Bioluminescence Resonance Energy Transfer (BRET)-based technology

His lab develops BRET- and FRET-based biosensors that produce different conformational outputs depending

Monitor GPCR internalization in real time with pHSense™ – no-wash, TR-FRET reagents from Revvity. Unlock microscopy-free real-time GPCR internalization with pHSense pHSense™ probes are optimized reagents With formats including TR-FRET, radioligand binding, cAMP, IP-One, and phospho-protein assays (ERK, AKT Ligand binding Measure receptor engagement with high sensitivity using TR-FRET, radioligand, and Fluorescent Arrestin Recruitment Detect β-arrestin recruitment with TR-FRET and luminescence assays tailored for

receptor trafficking and recycling, and more recently the cannabinoid entourage hypothesis — combining BRET-based depending on receptor tone Quantitative pharmacology as rescue operation — using operational models and BRET-based

Working on chemokine receptor CCR1 in multiple myeloma, free fatty acid receptor FFA2 in type 2 diabetes Her lab studies chemokine receptor CCR1 in multiple myeloma and cancer-to-bone metastasis, free fatty why orphan receptor targeting may not require knowing the endogenous ligand Receptor dimerization - BRET - variant receptors, the Hauser paper, and what industry has not yet done with existing drugs 39:43 BRET

structure/function relationships of GPCRs using live-cell bioluminescence resonance energy transfer (BRET her undergraduate studies, she has used computational methods like molecular dynamics simulations and free

To study GPCR signalling, they develop and use innovative optical methods based on FRET and single-molecule

structure/function relationships of GPCRs using live-cell bioluminescence resonance energy transfer (BRET

Michel Bouvier's lab on BRET-based conformational readouts.

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Choosing that problem required betting a career on a concept that the field's own architects considered The Vietnam War draft, the NIH, and the two-year assignment that redirected a career 14:02 The Yellow Berets

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Bruns Fred Bruns discovered the first positive allosteric modulator (PAM) of a GPCR in the late 1980s Fred obtained an A.B. in Psychology from Washington University in St.