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assay and Kd= 42nM in Tag-lite®). Competition experiments of binding of fluorescent ligand CELT-335 to living HEK-293 T cells expressing W.; Wu, Y.; Zhao, S.; Shui, W.; Li, S.; Korde, A.; Laprairie, R. B.; Stahl, E. L.; Ho, J. Cell   2016 , 167  (3), 750-762.e14. https://doi.org/10.1016/j.cell.2016.10.004 . (5)         Li, X.; A Fluorescent Ligand-Binding Alternative Using Tag-Lite® Technology.

cropped region, not a patch of fluorescence — the entire pancreatic islet , 100–200 microns across, lighting The Moment GPCR Imaging Became a Turning Point Before the islet lit up, the collaboration wasn’t even A ligand-based peptide probe could, offer the consistency and surface selectivity GPCR imaging demands The islet lighting up wasn’t the pinnacle. It was the proof of concept. If JB's story resonates 🎧 Listen to part 1 of this series with Dr.

Live-cell high-content screening (HCS) offers an increasingly valuable alternative. By quantifying ligand–receptor interactions directly in intact cells, HCS allows researchers to observe Using HEK-293 cells stably expressing CB2R, fluorescent tracers such as CELT-331 can report on ligand HCS can support CB2 ligand discovery: 1. CB2 ligand characterization.

2,3,*, Analisa Thompson Gray 2, Feyisayo Adegboye 2, Ainsley McDonald-Boyer 3, Fubiao Shi 1, Dianxin Liu 1, Jose Maldonado 5, Jiesi Feng 4, Yulong Li 4, Simon Alford 5, Julio E. The Department’s strengths lie in GPCR signal transduction and neuroscience, and she has expanded it She gave the Fritz Lipmann Lecture at ASBMB in 2001. " Heidi Hamm on the web The Hamm Lab Vanderbilt School of Medicine Pubmed Google Scholar LinkedIn Twitter Dr.

<< Back to podcast list Strategic Partner(s) Dr. Nicolas Gilles About Dr. Nicolas Gilles "Dr. His strongest expertise lies in therapeutic target identification and all the steps from venom manipulations When the pharmacological properties of these new ligands are deemed exceptional, a lead optimization Nicolas Gilles on the web Google Scholar LinkedIn Dr. GPCR Enjoying the Dr. GPCR Podcast? Articles Asking Better Questions in Science: A Practical Guide for Emerging Researchers When the Islet Lit

For decades, molecular modeling focused on explaining — rationalizing why a ligand binds, why a mutation With tools like AlphaFold , his lab can now model receptor–peptide complexes in seconds — an achievement A good model doesn’t just match published structures; it forecasts new biology, new ligands, and new That’s where the future of GPCR research lies — not in describing the past, but in anticipating it.