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Using time-resolved cryo-electron microscopy (cryo-EM) and variability analysis to monitor the transitions This detailed process provided by the cryo-EM study offers a clear view of these dynamic states, from This highlights the power of advanced imaging techniques like cryo-EM in solving complex biological puzzles Time-resolved cryo-EM of G-protein activation by a GPCR. Nature (2024).

August 2022 "Single particle cryogenic-electron microscopy (cryo-EM) is used extensively to determine Although a similar strategy has the potential to broadly facilitate cryo-EM structure determination of The fusion strategies explored here are likely applicable to cryo-EM interrogation of other GPCRs and

Electron cryo-microscopy (cryo-EM) is a powerful technique for the structural characterization of biological In recent years, pharmaceutical companies have begun to utilize cryo-EM for structure-based drug design As a result, cryo-EM has begun to make major impacts in bringing critical therapeutics to market. In this review, we discuss recent instructive examples of impacts from cryo-EM in therapeutics design We also discuss the opportunities afforded by emerging technological advances in cryo-EM, and the prospects

work has produced structural data at the extracellular domain level and, more recently, full-length cryo-EM Her work employs X-ray crystallography, single-particle cryo-EM, mutagenesis, and mass spectrometry, same year, established inactive, active, and intermediate conformational states of this receptor using cryo-EM inside the transmembrane bundle: a discovery nobody expected When Fan's team solved the full-length cryo-EM The field has moved from not knowing the subunit composition of GABA-B to holding cryo-EM structures

mechanism of this family of G protein-coupled receptors using innovative approaches, including biosensors, cryo-EM

Yamina Berchiche emphasizes the importance of diverse voices in the GPCR community: “It’s been a very Allosteric Modulation: The New Frontier of GPCR Drug Discovery Ben reflects on the emerging potential Structural Biology Roadblocks: The Cryo-EM Challenge One bottleneck in Ben’s work is visualizing binding sites of PAMs via cryo-EM , due to low compound potency and membrane-embedded binding pockets. Still, by collaborating with structural biology teams and combining cryo-EM with NMR , his lab is narrowing