Dr. GPCR Spotlights Revvity’s pHSense™ Internalization Tools
- Dr. GPCR News
- Sep 22
- 2 min read
A breakthrough GPCR internalization assay now featured in the Dr. GPCR Ecosystem
Boston, MA – September 2025 — Dr. GPCR, the global nonprofit platform dedicated to advancing GPCR science through education, community, and platform visibility, is proud to spotlight pHSense™, a new reagent family from Revvity, now featured across the Dr. GPCR Ecosystem.
Developed to address long-standing challenges in GPCR internalization assays, pHSense™ reagents combine live-cell, no-wash protocols with time-resolved fluorescence (TRF) detection, enabling real-time detection of GPCR internalization—without microscopy.
“The day we saw dose-dependent internalization in endogenous GLP1R cells—without microscopy—that was the turning point,” said Dr. Eric Trinquet, Director of Research and Development at Revvity.
A GPCR Internalization Tool Designed for Real Research Needs
Built on more than two decadeA GPCR Internalization Tool Designed for Real Research Needss of GPCR assay innovation, pHSense™ was developed to overcome three persistent barriers in internalization studies:
Complex imaging workflows
Limited scalability for high-throughput screening
Low sensitivity in detecting endogenous GPCRs
With pHSense™, scientists can finally track GPCR internalization in real time—even at physiological expression levels—using a simple plate-based format.
The reagents are compatible with HTRF readers and validated in GLP1R and Mu opioid receptor (MOR) models.
Part of a Complete GPCR Reagent Portfolio
pHSense™ is the latest addition to Revvity’s GPCR reagent portfolio, which supports every stage of the signaling cascade:
GPCR ligand binding – TR-FRET, radioligand, Tag-lite®
G-protein activation – cAMP, IP-One, GTP assays
β-arrestin recruitment assays
Downstream readouts – phospho-ERK, AKT, CREB, MEK and other phoshoproteins
These reagents are optimized for high-throughput GPCR screening, reproducibility, and translational depth.
🎙️ Hear More About pHSense™ on the Dr. GPCR Podcast
To mark the spotlight, Dr. GPCR invited Dr. Eric Trinquet to the Dr. GPCR Podcast, where he shares the story behind pHSense, including its application in real-time internalization assays and the scientific “aha” moments that drove its development.
“This isn’t just another product,” said Dr. Yamina Berchiche, Founder of Dr. GPCR. “It’s a leap forward in how the community studies GPCRs at scale and in context.”
🔗 About Dr. GPCR
Dr. GPCR is a global nonprofit platform advancing GPCR research through education, community, and platform visibility. Through our podcast, training programs, and partner content, we help scientists connect, collaborate, and innovate in the world of G protein-coupled receptors.
🔗 About Revvity
Revvity is a global life sciences company delivering translational tools and diagnostics. Its GPCR research tools support discovery teams and academic labs with precision reagents and validated assay platforms.
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