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Crystal structures suggest ligand access to the orthosteric binding site of MT1 and MT2 receptors through entry route for 2-iodomelatonin, a nonselective agonist with a slower dissociation rate from the MT2 receptor which revealed different trajectories passing through the gap between TM helices IV and V for both receptors The side-chain flexibility of Tyr5.38 was significantly different in the two receptor subtypes, as assessed is a way of connecting the orthosteric binding site and the membrane core for lipophilic melatonin receptor

“This wasn’t with much foresight for a couple years down the road.

August 2022 "The atypical chemokine receptor 1 (ACKR1) was discovered on erythrocytes as the Duffy blood group antigen ( Cutbush et al., 1950 ), also called Duffy-antigen/receptor for chemokines, or DARC (

by sea bass gonadotropin-inhibitory hormone peptides in COS-7 cells transfected with their cognate receptor significantly decreased forskolin-elicited CRE-luc activity in COS-7 cells transfected with their cognate receptor Notably, GnIH2 antagonized Kiss2-evoked CRE-luc activity in COS-7 cells expressing GnIHR and Kiss2 receptor

Angiotensin 1-7 (Ang-1-7), an endogenous peptide, acts at the G protein coupled MAS1 receptors (MASR)

In this session, you’ll gain: ✅ Proven strategies  to balance in vitro vs. in vivo testing early — when The real friction point lies downstream : translating receptor–ligand interactions into actionable development “GPCRs are nature’s prototype allosteric proteins. Everything they do is allosteric.”

Questions about how opioids impair breathing, why xylazine complicates interventions, and how receptor-level This approach makes her models not just rigorous, but translational—bridging the gap between receptor She is especially interested in mu-opioid receptor signaling  and how xylazine, as an alpha-2 adrenergic That personal loss transforms complex receptor pharmacology into something immediate and human.

What if you could directly measure receptor internalization in physiologically relevant cells without These probes become brighter and have a longer lifespan as internalized receptors enter acidic endosomes—translating his team presented a data set that transformed everything: a clean, dose-dependent response of GLP-1 receptor There is excitement about combining pHSense with other HTRF assays for multi-pathway mapping—G protein

Bigger Picture: GPCR Science Meets Public Health At its core, Catherine Demery’s research  is about receptors and signaling pathways—how mu-opioid  and alpha-2 adrenergic receptors  interact to disrupt breathing Fentanyl, through the mu-opioid receptor, blunts the brainstem’s inspiratory drive so that each breath By displacing opioids from the mu-opioid receptor, it restores breathing within minutes. But that mechanism has no impact on xylazine, which works through alpha-2 adrenergic receptors.

Breakthroughs this week: New work clarifies how active-state GPCR conformations can support coupling Must-read publications:  Studies on active-state GPCR ensembles and their transducer coupling, biased angiotensin receptor ligands, and circuit-selective analgesia in pain models. The Revvity team asked a harder question: What if you could measure receptor internalization in native Subtype specificity:  selectively track receptor subtypes in complex brain tissue.

When pharmacologists misinterpret how an antagonist interacts with its receptor, the consequences ripple But if the antagonist binds tightly and dissociates slowly, the receptor remains blocked, even at high Antagonist “hogs” the receptor, depressing the response, even if more agonist is added. Common misconceptions  arise when irreversible binding, receptor reserve, or allosteric effects mimic He challenges the idea that curve shape alone is diagnostic, pointing out how features like receptor

While some receptors selectively activate specific G protein families, others are more versatile, yielding diverse responses based on cell-specific G protein expression. Apart from G proteins, GPCRs engage other effectors for signaling modulation. Originally recognized for inhibiting G protein signaling, they also influence specific pathways such G. 2021).

Kathleen M Caron and her team studied the GPER/GPR30 complex with β1-adrenergic receptor and AKAP5 in in auditory neurons GPCRs in Oncology and Immunology G protein-coupled estrogen receptor (GPER)/GPR30 forms a complex with the β1-adrenergic receptor, a membrane-associated guanylate kinase (MAGUK) scaffold protein, and protein kinase A anchoring protein (AKAP) 5 in MCF7 breast cancer cells Reviews, GPCRs, and Exhibition June 2 - 7, 2024 | Chemotactic Cytokines June 9 - 14, 2024 | 2024 Phosphorylation and G-Protein

GPCR Activation and Signaling Ligand-induced activation and G protein coupling of prostaglandin F2α receptor Plasma membrane preassociation drives β-arrestin coupling to receptors and activation. An allosteric modulator of the adenosine A1 receptor potentiates the antilipolytic effect in rat adipose GPCRs in Oncology and Immunology DANGER Signals Activate G-Protein Receptor Kinases Suppressing Neutrophil Mechanism of Activation of the Visual Receptor Rhodopsin.

It began with an unmet need: how to track Gq-coupled GPCR activity without the mess of calcium flux or shown in beta cells 🚀 2025: Revvity launches pHSense A Day That Changed Everything: The Endogenous Receptor s second “aha” moment with pHSense came the day his team showed internalization of endogenous GLP-1 receptors It validated the broader goal: giving scientists tools to study receptors in their native, unmodified

Can we leverage structural and computational insights not just to explain receptor–ligand interactions Instead of rehashing how a known ligand binds to a known receptor, they aim to produce predictions that proteins and arrestins. For dopamine receptor studies, they built predictive models before experimental structures were available It struggles with ligand-bound conformations, GPCR–G protein complexes, and receptor–peptide interactions

Agonist-to-Antagonist Conversion: Enabling the Design of Nucleoside Functional Switches for the A2A Adenosine Receptor "Modulators of the G protein-coupled A2A adenosine receptor (A2AAR) have been considered promising agents Structural analysis revealed that the introduced thiophene modification restricted receptor conformational This approach can expand the repertoire of adenosine receptor antagonists that can be designed based

Reflex arcs, compensatory pathways, and receptor trafficking can turn your expected outcome on its head red flags early and make course corrections before trials derail. ✅ Practical strategies  for using receptor Dobutamine’s dual action on beta and alpha receptors, for example, invites reflex bradycardia that blunts Internalized Signaling: Same Receptor, Different Story A GPCR response isn’t always over when the receptor In this module, you’ll explore how some receptor–agonist complexes continue signaling from endosomes,

September 2022 "Melanocortin 4 receptor (MC4R) is part of the leptin-melanocortin pathway and plays an We have previously reported that the MC4R forms homodimers, affecting receptor Gs signaling properties NanoBRETTM studies to determine protein–protein interaction were conducted, confirming decreased homodimerization capacities of chimeric receptors in HEK293 cells. Gq/11 signaling of chimeric receptors was analyzed using luciferase-based reporter gene (NFAT) assays

2022 N-terminal alterations turn the gut hormone GLP-2 into an antagonist with gradual loss of GLP-2 receptor selectivity towards more GLP-1 receptor interaction "Background and purpose: To fully elucidate the regulatory role of the GLP-2 system in the gut and the bones, potent and selective GLP-2 receptor (GLP To examine selectivity, COS-7 cells expressing human GLP-1 or GIP receptors were assessed for cAMP accumulation

2022 "Abstract Rodent models are commonly used to evaluate parathyroid hormone (PTH) and PTH-related protein Divergence, however, in the amino acid sequences of rodent and human PTH receptors (rat and mouse PTH1Rs are 91% identical to the human PTH1R) can lead to differences in receptor-binding and signaling potencies PTH1R replaces a segment (exon 4) of the murine PTH1R gene so that the human and not the mouse PTH1R protein

2022 In vitro assays for the functional characterization of (psychedelic) substances at the serotonin receptor More specifically, this review covers assays to monitor the canonical G protein signaling pathway (e.g . measuring G protein recruitment/activation, inositol phosphate accumulation, or Ca2+ mobilization), assays to monitor non-canonical G protein signaling (such as arachidonic acid release), assays to monitor β-arrestin recruitment or signaling, and assays to monitor receptor conformational changes.

Traditionally, the most reliable and commonly used method is the amide coupling  using acid and amine  is the most suited for bioconjugation with proteins, DNA, etc, thanks to its reaction with the free Its biggest advantage is the presence of Cys residues in proteins, although sometimes S-S bridge reduction G.; Boström, J. D.; Reid, R.; Robinson, L.; Ashley, G. W.; Santi, D. V.

, and Antonios Kolocouris refine AlphaFold models using binding calculations for human adenosine A3 receptor News from January 22nd to 28th, 2024 GPCR Activation and Signaling Optical Control of Adenosine A2A Receptor interactions in Penaeus vannamei and Vibrio parahaemolyticus Involvement of Protease-Activated Receptor2 receptors via engineered Gt and Nb35 application Imaging GPCR Dimerization in Living Cells with Cucurbit and Exhibition June 2 - 7, 2024 | Chemotactic Cytokines June 9 - 14, 2024 | 2024 Phosphorylation and G-Protein

They’re kinetic game-changers —compounds that rewrite the relationship between ligand , receptor , and Understanding how persistent binding affects receptor turnover, tissue penetration, and PK/PD relationships This PK/PD dissociation  means: Drug exposure may end, but receptor occupancy remains. get released next month, featuring real questions from discovery scientists tackling enzyme kinetics, receptor Corner & Secure Your Spot for the October 30 AMA Why Terry’s Corner Pipeline risk isn’t just at the receptor—it

Considering the central role of the serotonin 5-HT2A receptor in the distinct effects of psychedelics

September 2022 Fentanyl activates ovarian cancer and alleviates chemotherapy-induced toxicity via opioid receptor-dependent

regulation of cancer cell cycle and metastasis "β-Arrestins are ubiquitously expressed intracellular proteins identified for their contribution to GPCR desensitization to agonist-mediated activation, followed by receptor now recognized that in addition to GPCR arresting (hence the name arrestin). β-Arrestins are adaptor proteins recruitment, activation, and scaffolding of numerous cytoplasmic signaling complexes and assist in G-protein receptor signaling, thus bringing them into close proximity.

  📚 This week’s paper highlights: GLP-1R/GIPR biased agonism enhances metabolic outcomes Ghrelin receptor flips D2 signaling without a ligand MOR-PAM shows G protein-selective bias   The insights are ready. Constitutive ghrelin receptor activity, not dimerization or ligand binding —reverses dopamine D2 signaling

GPCR Activation and Signaling Coupling between GPR143 and dopamine D2 receptor is required for selective potentiation of dopamine D2 receptor function by L-3,4-dihydroxyphenylalanine in the dorsal striatum Small-molecule targeting of GPCR-independent non-canonical G protein signaling inhibits cancer progression Gαi-derived peptide binds the µ-opioid receptor. A growing understanding of the role of muscarinic receptors in the molecular pathology and treatment