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To efficiently run these screening campaigns high throughput screening assays are used. How Fluorescence Polarization Assays Work: Principles and Applications in GPCR Research FP assays work FP assays are convenient and easy to manipulate. where they used CELT-419 for D3 dopamine receptor binding assays in baculoviruses. 3.       The future of fluorescence polarization assays is closely tied to fluorescent ligands.

During that project we tested its validity as a fluorescent probe for Tag-lite® assays, where we used a set of 7 cannabinoid ligands (both natural and synthetic) to validate and optimize the assay. It bears a hydrophilic fluorophore compatible with the terbium donor in TR-FRET assays. Figure 2.Assay Principle: TR-FRET assay using CELT-335, a dual (CB1/CB2) fluorescent ligand that serves Comparison of binding affi nities using radioligand binding or TR-FRET assays.

GPCRs in Neuroscience A bistable inhibitory OptoGPCR for multiplexed optogenetic control of neural circuits Highly multiplexed bioactivity screening reveals human and microbiota metabolome-GPCRome interactions

pHSense™ reagents enable real-time GPCR internalization detection A breakthrough GPCR internalization assay Developed to address long-standing challenges in GPCR internalization assays , pHSense™ reagents  combine Built on more than two decade A GPCR Internalization Tool Designed for Real Research Needs s of GPCR assay GPCR ligand binding  – TR-FRET, radioligand, Tag-lite® G-protein activation  – cAMP, IP-One, GTP assays β-arrestin recruitment assays Downstream readouts  – phospho-ERK, AKT, CREB, MEK and other phoshoproteins

It was simply a clean, scalable assay—and a wave of new possibilities. Instead of creating another black-box assay, they designed pHSense around rare-earth europium probes. This was the foundation of a new set of assay tools. Whether in industry or academia, whether overexpressing or not, you can adapt the assay to your system Build or adopt assays that can evolve with your questions—like pHSense.” What’s Next?

However, these conventional assays often provide limited information on intermediate signaling events (e.g G protein recruitment assay [6], mini-G recruitment assay [7], GRK and β-arrestin recruitment assays [8]), G protein activation (e.g TRUPATH assay [9]), receptor trafficking ( e.g FYVE assay [10]),  β-arrestin  YEN assay [13]), among others. Zhou, Y., et al., Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors. 

In this session, you’ll gain:   ✅ A framework for understanding when apparent multiple affinities really information from binding experiments that advance your work efficiently ✅ Clarity that helps you move from assay Move Faster, Smarter, and with Confidence   When you understand what your assays are truly showing you facilitates formation of ligand-receptor-G protein complexes —an observation that creates the illusion of multiple

Docking scores were meaningless without assays. For receptor assays and the biological interpretation of ligands, he relies on a network of collaborators Chemistry fuels assays. Assay data flows back into models. A pharmacologist who understands docking, or a modeler who knows assay constraints, will collaborate , and assay data cycling back to refine predictions.

ligand mechanism to the decisions that shape affinity, efficacy, selectivity, safety, and downstream assays That subtle distinction opened the door to a brand-new assay format. Instead of imaging-heavy workflows, pHSense offers a no-wash, plate-reader–ready, high-throughput assay Cleaner data:  higher signal-to-noise ratios sharpen CNS assays. Speed:  faster GPCR target validation and assay development.

“We don’t just deliver compounds, we solve assay problems.” — Dr. Democratize access to high-performance chemical probes and make assay development faster, cheaper, and workflow on the company page . _______________ Keyword Cloud:   GPCR data platform , fluorescent ligands , assay

you chain your highest-paid scientists to repetitive admin work, you’re not solving problems — you’re multiplying pipes instrument outputs into a central hub — where QC, analysis, consumption and consolidation across assays GPCR Drug Discovery The only way out for GPCR drug discovery programs isn’t more people or shinier assays The Hidden Costs of Poor Drug Discovery Data Management Stop pretending more hires or new assays will

Plus, Celtarys explores ligand selection for better assays, and co-founder Dr. Radioligands – Assay Smarter Celtarys explores how fluorescent ligands enable safer, high-throughput Learn how these tools help retain assay integrity while eliminating radioactivity—perfect for next-gen Maria Majellaro reveals how Celtarys evolved from a vision into a company solving real-world assay problems

fluorescently labeled ligands and innovative chemical biology tools to support real-time, non-radioactive GPCR assays GPCR partner, Celtarys will be featured across multiple touchpoints in the ecosystem, including the Dr labeled ligands, with excellent affinity and pharmacological profiles, designed to advance GPCR-targeted assay

Yamina’s Corner opens for strategic consulting, and our partner Celtarys unveils a robust TR-FRET assay Yamina’s Corner delivers GPCR consulting that cuts through the noise, designing assay cascades, setting Visit Yamina’s Corner Now CELT-335 - Celtarys Validates New Assay for CB1/CB2 Screening Dr. GPCR partner Celtarys Research has validated a TR-FRET assay for cannabinoid receptor ligands using their

Celtarys Research expands its assay tools with CELT-419 for D3 receptor studies, and Dr. translational PK/PD   Subscribe to The Kenakin Brief Fluorescent Probe CELT-419 Powers D3R Binding Assays Celtarys Research presents CELT-419, a nanomolar-affinity fluorescent ligand optimized for D3 receptor assays With strong signal stability, HTS compatibility, and broad assay versatility, CELT-419 is designed for

the cell ✅ Tools for evaluating scaffold permeability using modern, cost-effective pharmacokinetic assays In a traditional assay, they look the same. But in vivo? The good news: we now have reliable, low-cost assays to find out —Kenakin walks through the essential GPCRs Aren’t Just Surface Receptors Anymore From β2-adrenoreceptors to CCRs and dopamine receptors, multiple

Maria Majellaro, highlighting their fluorescent ligand tools for live-cell GPCR assays.   Maria Majellaro of Celtarys shares how her team translates medicinal chemistry into practical GPCR assay It emphasizes scaffold selection, linker optimization, and assay compatibility to enhance target binding

The assays are running, data is flowing in from your CROs or your internal labs , yet progress stalls This disconnect frequently leads to: Underperforming assays. I'll help write CRO scopes, meticulously review assay data, flag risks early, and keep your programs Systems That Drive Progress:  From assay tracking to data workflows, I design simple, scalable tools

Four hundred plates of assays, concentration-response curves, and mutagenesis screens are standard. _______ Keyword Cloud: GPCR scientist network, GPCR training program, mentorship in science, GTPγS assay

Corner you'll learn how model-based thinking helps you determine a drug’s mechanism of action and turn assay most difficult calls in pharmacology happen when two different mechanisms look identical in a single assay Without a framework, this looks like an assay error. In HIV entry studies, purified gp120 was too expensive for routine assays. A Case That Seemed Impossible In one real program, a compound produced four completely different assay

medicinal chemistry paved the way to co-founding Celtarys , a company now shaping the future of GPCR assay GPCR, is helping researchers worldwide gain access to customized, reliable assay tools without the delays

Every day spent misunderstanding what your assays are truly showing you can lead to costly missteps—wasted Kenakin’s kinetic insights help you translate assay readouts into actionable knowledge that keeps your ’t Let Outdated Models Slow Your Next Program The analytical tools that shaped traditional affinity assays You’ll gain clarity that accelerates your path from discovery to clinic: Know when your assays truly

When assays behave unpredictably, the wrong interpretation doesn’t just waste time; it costs viable compounds Career opportunities:  Postdocs in GPCR biophysics and assay development; industry scientist roles at Protect your pipeline:  Misinterpreting displacement curves in allosteric assays means discarding viable Avoid costly blind spots:  Discover how G protein stoichiometry can dictate whether your assay informs—or

previous posts, CELT-335, one of our fluorescent compounds, was successfully employed in a binding assay very useful in GPCR drug discovery, starting from hit and lead validation all the way to pre-clinical assays Reduced background noise: Improvements in signal-to-noise ratio are key in CNS assays. Faster assay development: also speeds GPCR target validation. A Robust and Efficient FRET-Based Assay for Cannabinoid Receptor Ligands Discovery.

that logic: Shared infrastructure, not duplicated equipment Centralized core facilities for receptor assays PhD students trained across multiple techniques, not just one pipeline Senior scientists embedded as The Power of GPCR Collaboration: Funding as a Force Multiplier Collaboration sounds warm and fuzzy. Instead of competing for multiple small grants, groups strategically pooled resources to build critical The Monash team built rotational PhD cohorts  — students cycled through multiple groups in their first

You can have the most brilliant minds and cutting-edge assays, but if your science isn't continuously My work isn't just  about the latest and best assay; it's about the framework that ensures the right assay data leads to the right decision. We'll look at how overlooked operational details, such as misaligned data from diverse GPCR assay types

I call it the Lego Bucket Problem : You’ve got CRO output, internal assay data, maybe even some promising arrives too fast and too fragmented CROs deliver output without clear integration Teams chase the wrong assays Here’s what I bring: Biology-First Strategy I’ve designed GPCR assays, led collaborations across research

Someone with strong assay development skills as well as strong data analysis and interpretation skills Mark:  “Are there particular assay types of interest?” Beth:  “We focus on biochemical, cell based, and radioligand binding assays to enable SAR, MOA, lead

Early In Vivo Wins the Race When is the right time to move beyond cell assays? In vitro work is invaluable for mechanistic understanding—assay volume control, expression system contrasts Modern real-time assays can deliver these insights earlier, faster, and cheaper than most teams assume Teams that proactively define experimental nuances early avoid receiving “perfectly executed wrong assays

visualizing intermediate states with triple-color FRET, and deorphanizing targets using the new G(z)ESTY assay G(z)ESTY as an optimized cell-based assay for initial steps in GPCR deorphanization .