Search Results

Celtarys Research expands its assay tools with CELT-419 for D3 receptor studies, and Dr. fuel confidence and fluency Move beyond theory to apply selectivity, ADME, and early safety in real development translational PK/PD   Subscribe to The Kenakin Brief Fluorescent Probe CELT-419 Powers D3R Binding Assays Celtarys Research presents CELT-419, a nanomolar-affinity fluorescent ligand optimized for D3 receptor assays With strong signal stability, HTS compatibility, and broad assay versatility, CELT-419 is designed for

(e.g G protein recruitment assay [6], mini-G recruitment assay [7], GRK and β-arrestin recruitment assays [8]), G protein activation (e.g TRUPATH assay [9]), receptor trafficking ( e.g FYVE assay [10]),  β-arrestin  YEN assay [13]), among others. pharmacological activity, allowing for the determination of drug kinetics—a critical aspect in drug development Some innovative solutions have been the development of the BERKY and the ONE-GO biosensors, designed

programs don’t fail because of weak molecules—they fail because biology behaves differently than the assay His work shows why chemical design can outperform antibodies and how rigorous assay validation bridges But HCS only works when assays are built with rigor and powered by the right fluorescent ligands. What You’ll Learn Why traditional radioligand assays miss critical spatial and kinetic signals Five phases a robust, reproducible HCS pipeline How fluorescent ligands strengthen specificity, relevance, and assay

Docking scores were meaningless without assays. For receptor assays and the biological interpretation of ligands, he relies on a network of collaborators Chemistry fuels assays. Assay data flows back into models. A pharmacologist who understands docking, or a modeler who knows assay constraints, will collaborate , and assay data cycling back to refine predictions.

Plus, Celtarys explores ligand selection for better assays, and co-founder Dr. Radioligands – Assay Smarter Celtarys explores how fluorescent ligands enable safer, high-throughput Learn how these tools help retain assay integrity while eliminating radioactivity—perfect for next-gen Maria Majellaro reveals how Celtarys evolved from a vision into a company solving real-world assay problems

Using Fluorescent Ligands Over Antibodies in Flow Cytometry Assays: Key Advantages These are key limitations These limitations can impact data quality, reproducibility, and assay flexibility. Selection for GPCR-Targeted Flow Cytometry The fluorophore tag is a key part of the flow cytometry assay Tag brightness and stability:  the brighter and more stable the better the assay outcome. 2.       CELT-240 in flow cytometry binding assays is suitable to measure the affinity of compounds for the D2

Corner, Yamina's Corner, biotech, systems thinking, signal transduction, operational excellence, drug development

The assays are running, data is flowing in from your CROs or your internal labs , yet progress stalls This disconnect frequently leads to: Underperforming assays. I'll help write CRO scopes, meticulously review assay data, flag risks early, and keep your programs Systems That Drive Progress:  From assay tracking to data workflows, I design simple, scalable tools

This emphasis may have caused other signaling pathways to be overlooked due to a need for adequate assay This further underscores the need for advanced assay technologies and a deep understanding of cellular Recently, several high-throughput screening (HTS) assays for ERK activation have been developed, each Each assay offers unique advantages for detecting ERK activation, contributing to the broader toolkit In summary, targeting GPCR-activated ERK pathways represents a promising strategy for developing new

Sometimes when you’re in the field you forget the importance GPCRs holds in drug development as a whole After all, a good tool makes a good assay, and a good assay improves research capacity. She gave us an overview of the drugs she has participated in developing throughout her career, highlighting Xiaoyu Zhang showed great insight into new ligands for new E3 ligases for PROTAC development.

GPCR – Precision Tools for GPCR Assays Dr. GPCR and Celtarys Research have teamed up. Sakmar Lab’s Toolkit Goes Public The latest podcast features a discussion about a scalable GPCR-RAMP assay

Dual-epitope tagged constructs (N-term FLAG, C-term 1D4) Compatible with multiple readouts: proximity, immuno assays ______ Keyword Cloud : GPCR scientist network , GPCR online course , GPCR data platform , multiplex assays

The Tools to Detect Them Using the multiplexed GPCR library and Luminex assay , researchers can now: GPCR ecosystem , multiplex assays

Four hundred plates of assays, concentration-response curves, and mutagenesis screens are standard. _______ Keyword Cloud: GPCR scientist network, GPCR training program, mentorship in science, GTPγS assay

Corner you'll learn how model-based thinking helps you determine a drug’s mechanism of action and turn assay most difficult calls in pharmacology happen when two different mechanisms look identical in a single assay Without a framework, this looks like an assay error. In HIV entry studies, purified gp120 was too expensive for routine assays. A Case That Seemed Impossible In one real program, a compound produced four completely different assay

Every day spent misunderstanding what your assays are truly showing you can lead to costly missteps—wasted Kenakin’s kinetic insights help you translate assay readouts into actionable knowledge that keeps your ’t Let Outdated Models Slow Your Next Program The analytical tools that shaped traditional affinity assays You’ll gain clarity that accelerates your path from discovery to clinic: Know when your assays truly

I call it the Lego Bucket Problem : You’ve got CRO output, internal assay data, maybe even some promising arrives too fast and too fragmented CROs deliver output without clear integration Teams chase the wrong assays Here’s what I bring: Biology-First Strategy I’ve designed GPCR assays, led collaborations across research

They design assays differently. They interpret deviations differently. Dual-assay strategies (high and low sensitivity) are essential, not optional. Assay Volume Control: Classification Through Contrast Sensitivity doesn’t merely change the size of the ligands from a single system: The same molecule can occupy different mechanistic categories across assay requires understanding where the ligand sits on the operational curve—not just where it sits in one assay

pipes instrument outputs into a central hub — where QC, analysis, consumption and consolidation across assays GPCR Drug Discovery The only way out for GPCR drug discovery programs isn’t more people or shinier assays The Hidden Costs of Poor Drug Discovery Data Management Stop pretending more hires or new assays will

visualizing intermediate states with triple-color FRET, and deorphanizing targets using the new G(z)ESTY assay G(z)ESTY as an optimized cell-based assay for initial steps in GPCR deorphanization .  

Dates:  October 3 to 31, 2024 What You’ll Master during the five sessions: Utilizing new cellular assays GPCRs and mRNA antigens enable drug discovery and development Mapping GPCR-RAMP complexes with MolBoolean Screening GPR75 Modulators Using Medium-Throughput Reporter Gene Assay GPCR Therapeutics Optimizes the Joint Development of New Drugs Using Intralinks’ Virtual Data Room   GPCR Events, Meetings, and Webinars 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

Elements of a comprehensive and effective GPCR discovery Master advanced applications: Using new cellular assays codon-optimized clytin II gene in Chinese hamster ovary-K1 cells and its use in the G-protein-coupled receptor assays GPCR Events, Meetings, and Webinars September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

activation state of G protein-coupled receptors are a useful addition to the molecular pharmacology assay the detection of both (partial) agonism and inverse agonism on living cells in a microplate reader assay better correlated with binding affinity values that were measured in radioligand competition binding assays biosensor in membranes might be a ready-to-use, high-throughput alternative for radioligand binding assays that in addition can also detect ligand efficacies with comparable values as the intact cell assay."

If you’re applying orthosteric logic to modulator-driven systems, you’re likely misreading your assays—and Binding assays  report on one set of receptor states. Functional assays  track another.

the cell ✅ Tools for evaluating scaffold permeability using modern, cost-effective pharmacokinetic assays In a traditional assay, they look the same. But in vivo? The good news: we now have reliable, low-cost assays to find out —Kenakin walks through the essential

membrane-driven modulation of mGluR2, and a big headline, Novo Nordisk’s $2.2B deal with Septerna to develop In Episode 166, Ben Clements dives into opioid pharmacology, GPCR-targeted PAMs, and how old-school assays

Ligand-directed labelling strategies  are a newly developed way to attach a fluorescent tag to a GPCR other ligands and are very interesting in TR-FRET (Time-Resolved Förster Energy Resonance Transfer) assays A Robust and Efficient FRET-Based Assay for Cannabinoid Receptor Ligands Discovery.

8 and colon formation, and cell migration and invasion were detected by wound healing and transwell assays Western blot and RT–qPCR assays were carried out to measure the downstream factors of the interaction The effect of CCL25 on the development of SACC in vivo was examined by a xenograft tumor model in nude

analogies (think batteries and balance scales), Terry reveals why different tissues—and even different assays—can

Embark on this week’s thrilling updates in GPCR research, learning opportunities, and industry developments Topics: New cellular assays, real-time kinetics, and unique GPCR behaviors. Exclusive Deal for Scientists Residing and Working in Developing Nations If you live and work  in a developing -3, 2024 Join leading scientists to investigate the most recent advancements in GPCR-targeted drug development 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays