Search Results

Tom Sakmar, Emily Lorenzen, and Ilana Kotliar about creating a multiplex system with DUET-tagged constructs

article GPCR Publication Highlights   GLP-1R/GIPR biased agonism enhances metabolic outcomes —with dual

Dual control: not just brightness but fluorescence lifetime, with drastic shifts as pH drops. Tag-lite development 🧪 Mid-2010s: Rare-earth scaffold work begins 🔬 2023: pHSense probes optimized for dual It’s the product of layered collaborations—with Durham University chemist David Parker on the probe chemistry

In this session, you’ll gain: ✅ A clear map of how drugs interact with enzymes before, during, and after

But a pattern emerged: some scaffolds showed dual responsiveness to pH through lifetime and brightness Mini Timeline: The Road to pHSense → Initial lanthanide probe design with Durham University (chemistry ) → Discovery of pH-sensitive dual response (brightness + lifetime) → Application to GPCR models with Revnity’s partnership with Durham (chemistry) and the Institute of Functional Genomics in Montpellier

If your team is arguing about “potency vs. effect size vs. duration,” this is your playbook for aligning Working closely with Professor David Parker of Durham University, Trinquet’s group cracked the scaffold

A new contributor article from our friends at Celtrays Research outlines how dual-labeled fluorescent Dual-label specificity blocks promiscuous ligand confusion Lanthanide donors + d2 acceptors = high SNR

Dobutamine’s dual action on beta and alpha receptors, for example, invites reflex bradycardia that blunts

GPCR and global experts at the GPCR Drug Discovery session during Discovery on Target 2025—where groundbreaking

Transform Flow Cytometry in GPCR Analysis GPCRs are a tough target in traditional antibody staining due

The turning point came during her master’s program in pharmacogenomics.

A GPCR program can collapse—not because of bad science, but because brilliant teams are forced into duct

Access this week’s key insight ➤ Dr GPCR Podcast – Decoding the Deadly Duo: Xylazine, Fentanyl, and Respiratory

on G protein coupling (efficacy) γ:  G protein’s effect on radioligand binding (efficacy of A) β:  Dual

This joint effort by both associations is repeated every year, with 2026’s being in Dublin, Ireland. Wendy Young’s talk, during session 3 on day 2, was also incredible. Maria alongside the other panelists during Wednesday's panel discussion. Finally, during Session 9 Prof. Now back in Europe, all she can think about is next year’s date, in Dublin, where she is sure she will

During that project we tested its validity as a fluorescent probe for Tag-lite® assays, where we used Figure 2.Assay Principle: TR-FRET assay using CELT-335, a dual (CB1/CB2) fluorescent ligand that serves

It also poses some disadvantages – just like acid-amine amide coupling, some byproducts are obtained during

better systems, but in reality, our most brilliant and expensive minds are stuck with low-impact tasks due

But because the program is run on duct tape and heroics instead of precision.

Competitive antagonism  occurs when agonist and antagonist re-equilibrate rapidly during the experiment

Their structures are hard to determine experimentally due to poor expression and the volatility of odorant

When does onset rate dictate therapeutic onset, and when does offset rate predict duration?

Learning from COVID-19 One striking example came from his COVID-19 work during his PhD.

Often, a low EC50 indicates high potency, but this isn't always the case due to various biological factors

Riding the GPCR Rollercoaster Early in his career at DuPont and BMS, GPCRs were red hot.

Ever wondered why a drug behaves like a miracle in one tissue and a dud in another?

Getting accepted into a PhD program during a personally difficult time.

Building for the Future The Sakmar lab built a system to meet that need: Dual-epitope tagged constructs

What emerged was a dual-tagged GPCR library  (DUET constructs), now publicly available via Addgene ,

From dual-epitope tagging to scalable multiplex assays, this conversation dives deep into the tools now