Search Results

In Episode 166, Ben Clements dives into opioid pharmacology, GPCR-targeted PAMs, and how old-school assays

used in drug discovery create large datasets regarding ligand synthesis and screening, ligand binding assays , signaling assays, cell imaging, protein structure determination, and omics applications, which can DL algorithms employ artificial neural networks with multiple layers of interconnected nodes which learn AI is providing a dramatic acceleration of the drug discovery process at multiple stages: 1. Unsupervised deep learning that takes advantage of large unlabelled data (e.g., millions of compounds and assays

Multiple subthreshold GPCR signals combined by the G proteins Gαq and Gαs activate the C. elegans egg-laying FREE Symposium - IPI Surfacing (June 15, 2023) Training School on “Cell-based assays to study Adhesion Antibody Engineering NEW Scientist—Immuno-Oncology Convergent Research - Senior Scientist, Cell-Based Assay

membranes: an alternative to radioligand binding methods "Background: Replacement of radioligand binding assays same order of magnitude but slightly higher than values obtained by the radioligand saturation binding assay

as a Novel TAAR1 Agonist: A Combination Strategy through Molecular Modeling Studies and Biological Assays Gene Therapy Suite Avantor® Expands Cellular Science Offerings with Multispan Cell Engineering and Assay AI 2023 Workshop Salipro Biotech AB Celebrates Milestones in CryoEM Structures and In Vitro Binding Assays

in blood and synovial tissue samples from RA patients, as well as to perform a functional migration assay Migration assays were performed using PBMCs isolated from these individuals in response to the synthetic

They employed a series of phospho-mimicking mutants and assays such as linear ion trap mass spectrometry , BRET, pull down, and transwell cell migration assays to investigate the effects of these modifications

However, cellular assays, molecular docking and site-directed mutagenesis studies have revealed that investigated whether the impact of point mutations, already shown to have deleterious effects on cellular assays

Methods & Updates in GPCR Research NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding FREE Symposium - IPI Surfacing (June 15, 2023) Training School on “Cell-based assays to study Adhesion - Antibody Engineering Scientist—Immuno-Oncology Convergent Research - Senior Scientist, Cell-Based Assay

Co-immunoprecipitation and pull-down assays confirmed interaction between NFα1/CPE and 5-HTR1E and 125I Cell survival assay in β-arrestin Knockout HEK293 cells showed that the NF-α1/CPE-5-HTR1E-mediated protection

Delve into new cellular assays, real-time kinetics, and unique GPCR behaviors. 🔥 Why You Should Enroll 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays Immunology Structural Basis for the Recognition of GPRC5D by Talquetamab, a Bispecific Antibody for Multiple

Topics: New cellular assays, real-time kinetics, and unique GPCR behaviors. 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

Methods Proliferation, migration and apoptosis assays were performed in ovarian cancer cells after fentanyl

FREE Symposium - IPI Surfacing (June 15, 2023) Training School on “Cell-based assays to study Adhesion - Antibody Engineering Scientist—Immuno-Oncology Convergent Research - Senior Scientist, Cell-Based Assay

Radioligand binding assays revealed considerable binding of kurarinone on D1R, D2LR, and D4R. Functional GPCR assays unfolded the compound's antagonist behavior on D1R (IC50 42.1 ± 0.35 μM) and agonist

of action for new GPCR ligands Essentials for effective GPCR discovery Discover: Advanced cellular assays 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

Previous assays verified that a typical G protein-coupled receptor, β-adrenergic-like octopamine receptor

Quantitative real-time probe-based polymerase chain reaction (qRT-PCR) assays are deployed in order to This can be deduced from the qRT-PCR assays; triggering CB1 receptors amplifies both NR4A1 and NR4A3

for the role of oligomerization in receptor signaling came from ensemble biochemical and biophysical assays

Methods of identifying and determining the levels of different chemerin forms in both mass and activity assays

Using a co-immunoimmobilization assay, we found that transient β2-AR dimers exist in a resting state,

Experimental approach: We used a series of behavioural assays to evaluate the acute responses of mice

Dates: October 3rd to 31st, 2024 Registrants will learn: The powerful applications of new cellular assays 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

Furthermore, in vitro and in vivo assays demonstrated that GA was dependent on GPR108 to exert anti-cancer

Using a co-immunoimmobilization assay, we found that transient β2-AR dimers exist in a resting state,

resistance, tune selectivity, and design molecules that adapt to real cellular environments—not just ideal assay Bring your toughest pharmacology questions — from receptor bias and assay design to enzyme kinetics —

tight mechanism with transcriptomic analysis revealing that while the majority of cancer cells express multiple Selective screening assays for ectopic ORs as well as investment on the structural characterization of ligands, characterization of the function of ORs in vivo, development of in vitro systems for functional assays

Registrants will learn: The powerful applications of new cellular assays to determine GPCR ligand behavior Modules: October 31st : The Eyes to See- The Importance of Pharmacologic Assays.

Mutant proteins were assayed to help reveal the basis of ligand specificity, and structural comparison

used for a range of different applications, including bioluminescence resonance energy transfer (BRET) assays