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Dates:  October 3 to 31, 2024 What You’ll Master during the five sessions: Utilizing new cellular assays Alzheimer's disease Nanoplastic at environmentally relevant concentrations induces toxicity across multiple GPCR-RAMP complexes with MolBoolean Screening GPR75 Modulators Using Medium-Throughput Reporter Gene Assay 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

If you’re applying orthosteric logic to modulator-driven systems, you’re likely misreading your assays—and Binding assays  report on one set of receptor states. Functional assays  track another.

This emphasis may have caused other signaling pathways to be overlooked due to a need for adequate assay This further underscores the need for advanced assay technologies and a deep understanding of cellular Recently, several high-throughput screening (HTS) assays for ERK activation have been developed, each Each assay offers unique advantages for detecting ERK activation, contributing to the broader toolkit By understanding the intricacies of GPCR signaling and utilising advanced assay technologies, researchers

opportunities:  Leadership-level roles in clinical operations and translational science, alongside multiple validate differences, confirm subtle shifts, and benchmark your results against literature or across assay Validate with confidence:  Benchmark your results against literature standards and confirm whether your assays

analogies (think batteries and balance scales), Terry reveals why different tissues—and even different assays—can

Whether you're heading into your first assay or trying to make sense of inconsistent data, Terry gives

activation state of G protein-coupled receptors are a useful addition to the molecular pharmacology assay the detection of both (partial) agonism and inverse agonism on living cells in a microplate reader assay better correlated with binding affinity values that were measured in radioligand competition binding assays biosensor in membranes might be a ready-to-use, high-throughput alternative for radioligand binding assays that in addition can also detect ligand efficacies with comparable values as the intact cell assay."

Every computational hypothesis moves downstream into experimental assays via close collaborations. Fast forward to today: dozens of GPCR structures  exist, many in multiple conformations or bound to G Matters Now The convergence of machine learning, improved GPCR structures, and scalable experimental assays

After all, a good tool makes a good assay, and a good assay improves research capacity.

Some responses—especially in calcium assays or complex tissue systems—depress the maximum agonist effect Calcium flux assays, though tricky, yield valid Schild plots when analysis excludes non-equilibrium maxima Caveat: Without multiple concentrations, competitive nature remains an assumption. pA₂ is an elegant

protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple In cellular-adhesion assays, LK30 specifically broke the trans-cellular interaction of ADGRL3 with teneurin

Kenakin walks through this as a living system , showing how GPCRs exist in multiple conformations and This has immediate implications for interpreting EC50 shifts, partial agonism, and assay readouts—especially The result is not just cleaner assay design but a sharper decision framework for selecting, prioritizing

activity (receptor binding affinity, G protein and arrestin signaling) in high throughput cell-based assays considerably more potent than the leading small molecule competitors in in vitro functional inhibition assays Figure 5: In an in vitro functional signaling assay on a human monocytic cell line, OB-004 demonstrated Figure 6: In a flow-based human monocyte endothelial transmigration assay, OB-004 strongly outperformed rapidly synthesize candidate hits from library-based screening so that they can be tested in cell-based assays

for their work on The N terminus-only (trans) function of the Adhesion GPCR Latrophilin-1 controls multiple Topics: New cellular assays, real-time kinetics, and unique GPCR behaviors. 🔥 Why You Should Enroll Engaging multiple sclerosis by drugging a “simple” GPCR. 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays position Adhesion GPCRs The N terminus-only (trans) function of the Adhesion GPCR Latrophilin-1 controls multiple

to replace inference with models that disentangle mechanism—so your next go/no-go, dose range, and assay Engineer assays on purpose:  Set ranges, controls, and system sensitivity to surface mechanism—before Assay flexibility:  Combine self-labeling tags with quantitative readouts (e.g., TR-FRET) to expand mechanism

While others pivoted, Sokhom went deeper: refining assays, exploring signaling bias, and building a robust

Leveraging decades of experience, their services include AI-driven target identification, target validation, assay platform, iPSC-derived models, optogenetics, and high-content screening techniques such as cell painting assays

Dates:  October 31, November 7 - 14 -21, and December 5, 2024 (five sessions) Topics:  New cellular assays to promote morning wakefulness LPA3 agonist-producing Bacillus velezensis ADS024 is efficacious in multiple 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

compounds to delineate the GPR15 receptor-mediated Gα protein signalling using a G protein activation assay and second messenger assay conducted on living cells. results show that the GPR15 receptor preferentially couples to Gi/o rather than other pathways in both assays

Whether you’re building assays or a scientific career, this is a must-read on why risk, surprise, and Biosensors make once-invisible dynamics assayable at scale. Read the roundup ➤ Why Dr.

tasked with building an in vitro pharmacology group from scratch , including infrastructure, hiring, assay

expression was evaluated at gene and protein level, the signalling pathway was investigated by an IP1 assay Cell migration was detected by the Boyden chamber migration assay, performed on macrophages derived from

other ligands and are very interesting in TR-FRET (Time-Resolved Förster Energy Resonance Transfer) assays A Robust and Efficient FRET-Based Assay for Cannabinoid Receptor Ligands Discovery.

Cryptic Sites, Longer Onset: Why Some Drugs Work Differently in Cells Than in Assays One of the most This has direct implications for: Assay timing and readout design Misclassification of lead candidates

Watch Episode 175 From failed assays to breakthroughs in GPCR modeling , Dr. Carlsson notes that it’s easier to find pharmacologists to run assays than it is to persuade chemists Trainees must learn how to interpret assay data, understand pharmacological context, and communicate

Terry’s Corner: Schild Analysis — Why It Matters Most assays show a clean rightward shift and we assume Who should listen Researchers navigating complex datasets, balancing innovation with assay rigor, or

We confirmed the interaction of VLGR1 with key proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells.

Topics cover new cellular assays, real-time kinetics, and unique GPCR behaviors. 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

8 and colon formation, and cell migration and invasion were detected by wound healing and transwell assays Western blot and RT–qPCR assays were carried out to measure the downstream factors of the interaction

mu-opioid receptor, positive allosteric modulators, GPCR training program, GPCR online course, GTPγS assay