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built numerous technologies in gene synthesis, DNA information storage, gene editing, and large-scale multiplexed assays.

Badr recounted Badr's Journey, Multiplexing Assays, and Collaboration Badr shared his learning process Yamina emphasized the potential of multiplexing assays in expanding the scope of scientific exploration

Ilana Kotliar built a multiplexed platform to map GPCR-RAMP interactions and uncover autoantibody-driven assay.” – Tom Sakmar After exploring several screening techniques, the lab adopted the Luminex bead-based assay , which allowed simultaneous detection of multiple GPCR-RAMP interactions. assays. focusing on a single receptor, students now gravitate toward platforms , biosensors , and data-rich assays

Assay Principle of Eurofins DiscoverX Cell-based Assays Whether you are developing small-molecule or assay types and applications to meet your specific GPCR research needs. with hundreds of binding and functional assays, available individually or in panels. These assays use the same platform as the Eurofins DiscoverX assays and beyond. These GPCRs are excellent for use in functional, structural, or identification assays.

conformations and allosteric probe dependence, and it can be revealed with the right combination of functional assays the biological basis of signaling bias, shows how to detect and quantify it using reliable functional assays Practical assay strategies, interpretation pitfalls, and the value of comparing pathway outputs quantitatively GPCR assays to obtain a better understanding of the ligand effects on the GPCR system. Their assays are industry-standard, accepted for regulatory potency testing, and backed by thousands

Explore Martin Marro’s impact on GPCR drug discovery, assay innovation, and translational pharmacology bridging academia, pharma, and biotech. << Back to podcast list Strategic Partner(s) GPCR Assay Strategy Marro’s career spans big pharma and biotech, encompassing functional assay development, GPCR internalization The conversation explores the realities of using fluorescence-based assays, the challenge of translating How advanced assay design is essential for translating cell-based GPCR signals to therapeutic outcomes

Trinquet discusses the science and story behind pH Sense, Revvity’s innovative GPCR internalization assay product R&D. << Back to podcast list Strategic Partner(s) From Rare Earth Probes to Internalization Assays Eric Trinquet What does it take to design a breakthrough GPCR assay—from idea to industrial impact? With a career spanning two decades at the intersection of fluorescence chemistry, functional assays, What shifted the team’s strategy from traditional calcium assays to IP1 accumulation—and why it mattered

Pfleger on NanoBRET live-cell assays, receptor complex pharmacology, and what it took to move GPCR biology those interactions reshape conformation, ligand binding, and signaling output in ways that isolated assays He developed BRET and NanoBRET-based live-cell assay platforms now widely adopted across the GPCR field Endpoint Assays Can Miss the Biology Entirely A single measurement at a fixed time point collapses all The problem is not the assay format itself; it is the assumption that one time point is enough. 2.

An assay measures a specific thing - not the biology you assume it measures One of Livingston's most consistently held principles: understanding exactly what your assay is measuring is not optional. Misinterpreting assay specificity is, in her view, one of the most consequential and underacknowledged technically disorienting and visually striking - a different relationship to data than any endpoint assay "You have to know what your assay is actually measuring.

Maria Waldhoer on why endpoint GPCR assays miss most of what ligands do — and what kinetic pharmacological Maria Waldhoer, CSO of InterAx Biotech AG, argues that endpoint assays — the field's default way of characterizing InterAx models receptor signaling pathways as systems of time-dependent equations, then runs kinetic assays , data The academic-to-Big-Pharma-to-biotech trajectory Key Insights from the Conversation Endpoint assays Standard assay kits that read cAMP at thirty minutes or arrestin recruitment at ten collapse time-resolved

Accelerated Program Progression: Design robust assay cascades and establish key go/no-go points to speed Accelerated Preclinical Progression I streamline critical operational processes, from advanced assay Small missteps can derail entire programs: underperforming assays, off-track CROs, and data that fails Before working with Yamina, we had solid internal momentum but needed to better align our biology, assay structure the screening funnel, define clear go/no-go criteria, and integrate ADME and mechanistic assays

Michelle Halls reveals how organized GPCR signaling drives assay innovation and new therapeutic insights This conversation is especially valuable for scientists developing functional assays, fluorescence-based What early cyclic AMP assays revealed about spatial signaling long before high-content technologies existed Why It Might Hit Home If you’ve ever: Faced unexpected assay behavior at ultra-low ligand concentrations Get behind-the-scenes conversations, advanced assay development strategies, and practical GPCR tools

Insights for GPCR researchers and functional assay innovators. << Back to podcast list Strategic Partner She shares approaches that combine genetic manipulation (CRISPR-Cas9) and functional behavioral assays Listeners interested in GPCR drug discovery, functional assay development, or fluorescence-based assays What functional and behavioral assays in mosquitoes reveal about the underlying diversity of GPCR signaling If you want to expand high-throughput or fluorescence-based assay strategies to non-traditional models

Discover pHSense™ portfolio From binding to signaling to internalization: Cell-based fluorescence assays This results in high sensitivity and specificity in no-wash, plate-based live-cell assays. pHSense™ Eu G-Protein Activation Quantify Gs, Gi, and Gq activation in real time with cAMP, GTP, and IP-One assays Arrestin Recruitment Detect β-arrestin recruitment with TR-FRET and luminescence assays tailored for Build Your GPCR Assay Workflow Meet the Team behind pHSense™ Eric TRINQUET Sr Director Life Sciences

He also discussed the evolution of the field, from biochemical radioligand binding assays to cell-based functional assays, and the influence of Terry Kenakin and chemical programs on his later work. They discussed strategies for analyzing large compound screening data, emphasizing robust assays and Arthur recounted a 2004 visit to a pharma company using replicates in assays. conversation highlighted the importance of establishing the correct disease context, setting up appropriate assays

I then undertook my first project where I demonstrated that MRAP2 regulates the signaling of multiple MRAP2 regulates GHSR1a signaling; this project brought forth our NanoBiT-based arrestin recruitment assay This powerful tool allowed us to create an assay that kinetically measured the arrestin recruitment to I joined the lab, I became proficient in many In-vivo techniques, I adapted my arrestin recruitment assay

Johannes Broichhagen reveals how fluorescent probes transform GPCR imaging, internalization studies, and assay He explains what chemical design can solve that antibodies can’t, how to validate functional assay systems , and why fluorescence-based assays paired with careful synthetic planning open doors for both high-resolution How parallel synthesis and side-by-side functional assays accelerate probe optimization and reduce false Tried to build assays that behave in living cells—not just on paper.

His focus is on expanding commercial reach and accelerating the adoption of the company’s GPCR assay Webinar Fluorescent Probes for GLP-1R and GIPR Imaging: From Cell Assays to In Vivo Systems Fluorescent Target Engagement for GPCR Drug Discovery The A₂A adenosine receptor NanoBRET® competitive binding assay In this article, we examine the assay principle, validation strategy, and performance across reference GPCR Functional Assay Fluorescent GTPγS enables sensitive, non-radioactive GPCR activity assays for drug

in collaboration with: Celtarys Research Fluorescent Probes for GLP-1R and GIPR Imaging: From Cell Assays Available across multiple spectral ranges for confocal, super-resolution, and intravital imaging. daLUXendin Broad GPCR fluorescent ligand portfolio across multiple receptor families Fluorescence Polarization,

During my PhD, I learnt radioligand binding assays, behavioral assays, autoradiography, stereotaxic surgeries

practical tools in the drug discovery toolbox The tractability of GPCRs as a pharmacological system — assay The Off-Rate That Saved a Program Standard binding assays showed identical affinity. The Neurocrin team had to confront an explanation no assay had been designed to find — and then built vanished entirely at the physiological vesicle pH of 5.5 — the pattern recurs: assays designed for convenience The practical knowledge of what buffer conditions work, what assay artifacts to expect, what the literature

Discover how Celtarys Research is transforming GPCR assay development with fluorescent ligands in this focus is on enabling fast, customizable development of fluorescent ligands and chemical probes for GPCR assays GPCR, Celtarys will bring those lessons to the broader community, providing insight into assay development Each probe must retain activity, remain soluble, and be compatible with a given assay format. Whether you're designing assays, developing probes, or seeking better tools for GPCR drug discovery,

INSERM with a specific interest in G protein-coupled receptors by developing original BRET and TR-FRET assays He discovered multiple rare and loss-of-function variants of the MT2 melatonin receptors that are associated

How academia and biotech collaborate to scale GPCR tools—covering fluorescence assays, GPCR internalization The episode explores gpcr internalization , fluorescence-based probe design, and how functional assay tools lose impact when distribution and access aren’t planned from the start Why fluorescence-based assays from academic tool development to real-world adoption Balancing innovation with validation in GPCR assay Her work centers on GPCR modulators, synthetic chemistry, and enabling robust biological assays through

Walk away with something you can apply in your next experiment, assay, or project decision. biological basis of receptor signaling bias and ligand-dependent conformations How to design functional assay panels that quantify bias rather than infer it Interpretation pitfalls and what assay system choice Whether you're deep in receptor biology or evaluating assay tools, these sessions are built for you. GPCR pharmacology, signaling, structural biology, allosteric modulation, biased agonism, assay design

David has over 20+ years of experience working in a drug discovery environment mainly in a specialist assay His current interests include the development of HTS fluorescence-based kinetic binding assays specifically

It is also a matter of G protein coupling, tissue context, and the signaling assay used to measure affinity microenvironment and adenosine-mediated immune suppression - why A2A and A2B are being targeted in oncology Assay Affinity is not a fixed property - it is assay-dependent The apparent affinity of adenosine for its own A cAMP assay and a binding assay using the same receptor and the same ligand can return different affinity A2B receptors at meaningful levels - a fact that complicates fluorescent ligand screening, binding assays

taste receptors in cancer and metabolic diseases, and emerging high-throughput methods for functional assay generation How computational approaches and wet-lab validation complement each other in functional assay localization and need to understand the mechanistic role of microdomains If you’re expanding functional assays

question through structure-guided drug discovery, biased ligand design, and high-throughput functional assays Roth is direct about this: with sufficient medicinal chemistry resources and modern assays, you can make Roth's lab leans heavily on BRET-based G protein and arrestin assays because they're relatively insensitive Tango and calcium assays are reserved for screening, where false negatives are the real cost. not uncertain" — the data Bryan won't talk about yet 47:13 Why BRET captures what second-messenger assays

Khorana’s lab made early key contributions and developed strategies to express, reconstitute and assay He also went on to discover a “counterion switch” in visual pigments and to develop strategies to assay Howard Hughes Medical Institute appointment, Tom advanced a series of novel biochemical and biophysical assay