Search Results

Whether you're heading into your first assay or trying to make sense of inconsistent data, Terry gives

Terry’s Corner: Schild Analysis — Why It Matters Most assays show a clean rightward shift and we assume Who should listen Researchers navigating complex datasets, balancing innovation with assay rigor, or Aside from his vast experience in drug development, not to mention his extensive publication record,

information from binding experiments that advance your work efficiently ✅ Clarity that helps you move from assay Move Faster, Smarter, and with Confidence   When you understand what your assays are truly showing you

Watch Episode 175 From failed assays to breakthroughs in GPCR modeling , Dr. Rather than focusing solely on explaining receptor behavior post hoc, his group began developing workflows Carlsson notes that it’s easier to find pharmacologists to run assays than it is to persuade chemists Trainees must learn how to interpret assay data, understand pharmacological context, and communicate

Learn how his research aims to develop models that can distinguish between active and inactive ligands

current study found that 133 of these receptors were also detected, highlighting the robustness of the assay previous study not detected in the current analysis, suggesting that variations in cell preparation and assay

proteins that participate in many vital physiological functions making them a popular target for drug development for the role of oligomerization in receptor signaling came from ensemble biochemical and biophysical assays Here, we review recent developments in single-molecule counting with a focus on photobleaching step counting

They employed a series of phospho-mimicking mutants and assays such as linear ion trap mass spectrometry , BRET, pull down, and transwell cell migration assays to investigate the effects of these modifications This knowledge could inform the development of therapeutic strategies aimed at targeting specific phosphorylation

as a Novel TAAR1 Agonist: A Combination Strategy through Molecular Modeling Studies and Biological Assays Gene Therapy Suite Avantor® Expands Cellular Science Offerings with Multispan Cell Engineering and Assay AI 2023 Workshop Salipro Biotech AB Celebrates Milestones in CryoEM Structures and In Vitro Binding Assays Genentech and NVIDIA Enter Into Strategic AI Research Collaboration to Accelerate Drug Discovery and Development

compounds to delineate the GPR15 receptor-mediated Gα protein signalling using a G protein activation assay and second messenger assay conducted on living cells. results show that the GPR15 receptor preferentially couples to Gi/o rather than other pathways in both assays

While others pivoted, Sokhom went deeper: refining assays, exploring signaling bias, and building a robust

to replace inference with models that disentangle mechanism—so your next go/no-go, dose range, and assay Engineer assays on purpose:  Set ranges, controls, and system sensitivity to surface mechanism—before Assay flexibility:  Combine self-labeling tags with quantitative readouts (e.g., TR-FRET) to expand mechanism

expression was evaluated at gene and protein level, the signalling pathway was investigated by an IP1 assay Cell migration was detected by the Boyden chamber migration assay, performed on macrophages derived from

of action for new GPCR ligands Essentials for effective GPCR discovery Discover: Advanced cellular assays therapy to prevent heart failure progression GPCR Binders, Drugs, and more Advances in GPCR-targeted drug development Announces EUR 60M Series B Financing to Advance a Pipeline of Novel GPCR-Modulating Therapies into Clinical Development 7, 2024 | 16th Annual PEGS Europe September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

used for a range of different applications, including bioluminescence resonance energy transfer (BRET) assays Here, we report the structure-based development of fluorescent ligands targeting the intracellular allosteric By means of these studies, we developed 14 as a fluorescent CCR2 ligand, enabling cell-free as well as

This has immediate implications for interpreting EC50 shifts, partial agonism, and assay readouts—especially The result is not just cleaner assay design but a sharper decision framework for selecting, prioritizing lets you predict safety margins, dose–response behavior, and translational risk before they derail development

Registrants will learn: The powerful applications of new cellular assays to determine GPCR ligand behavior Modules: October 31st : The Eyes to See- The Importance of Pharmacologic Assays. Exclusive Deal for Scientists Residing and Working in Developing Nations If you live and work  in a developing Advisory Board Generate:Biomedicines Announces Multi-Target Collaboration with Novartis to Discover and Develop Antibody-based Agonist and a Class A GPCR Regulating Hunger and Satiety OMass Therapeutics Expands Development

One potential solution is to develop analgesics that act at targets other than opioid receptors. Experimental approach: We used a series of behavioural assays to evaluate the acute responses of mice

In cellular-adhesion assays, LK30 specifically broke the trans-cellular interaction of ADGRL3 with teneurin - and ligand specific aGPCR functions using unique tools, and thus establishes a foundation for the development

We confirmed the interaction of VLGR1 with key proteins of MAMs by pull-down assays in vitro complemented by in situ proximity ligation assays in cells.

Cam Sinh Lu ,   Karen Gregory ,  Lauren May ,  Andrea Vernall , et al. for their fantastic paper on Development Registrants will learn: The powerful applications of new cellular assays to determine GPCR ligand behavior Modules: October 31st : The Eyes to See- The Importance of Pharmacologic Assays. Exclusive Deal for Scientists in Developing Nations If you reside and work in a developing country, complete therapeutics N-acyl glycines produced by commensal bacteria potentiate GLP-1 secretion as GPCR ligands Development

Cryptic Sites, Longer Onset: Why Some Drugs Work Differently in Cells Than in Assays One of the most This has direct implications for: Assay timing and readout design Misclassification of lead candidates

cruciferous vegetables, and insights into regulation of its physiological processes contribute towards the development the Octβ3 protein family, and an analysis using quantitative PCR showed that it was expressed at all developmental The injection of double-stranded RNA in an RNA interference assay indicated that PxOctβ3 regulates development

From dual-epitope tagging to scalable multiplex assays, this conversation dives deep into the tools now

Topics: New cellular assays, real-time kinetics, and unique GPCR behaviors. 🔥 Why You Should Enroll Exclusive Deal for Scientists Residing and Working in Developing Nations If you live and work  in a developing This technique for studying cell receptors could have sweeping implications for drug development DeepCure 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays of Neurological Disease s Activation of GPR55 alleviates neuropathic pain and chronic inflammation Developmental

Topics cover new cellular assays, real-time kinetics, and unique GPCR behaviors. 4th Transatlantic ECI GPCR Symposium September 18, 2024 | FREE Webinar - The value of GPCR cell-based assays

membranes: an alternative to radioligand binding methods "Background: Replacement of radioligand binding assays same order of magnitude but slightly higher than values obtained by the radioligand saturation binding assay

This Week’s GPCR Intelligence: From Set-Point Pharmacology to No-Wash Internalization Assays This week – Designing Drugs That Anticipate Physiological Pushback Most GPCR programs don’t fail because the assay

Whether you’re building assays or a scientific career, this is a must-read on why risk, surprise, and Biosensors make once-invisible dynamics assayable at scale. Read the roundup ➤ Why Dr.

the help of Luminex technology and collaborators at SciLifeLab in Sweden , the lab built a multiplex assay