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If you're interested in presenting a poster, there's still time. Therapeutics in the 23rd BioEquity Europe Conference Simon Bekker-Jensen and Mette M Rosenkilde received two proof

Homogeneous Time-Resolved Fluorescence (HTRF) is a TR-FRET-based assay[16] conducted in homogeneous conditions (12)      Martín-Fontecha, M.; Angelina, A.; Rückert, B.; Rueda-Zubiaurre, A.; Martín-Cruz, L.; Van De Time-Resolved Detection of Energy Transfer: Theory and Application to Immunoassays. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies:

Industry News Trevena Enrolls First Subject in TRV045 Proof-of-Concept Trial Evaluating S1PR Mechanism

surface protein in yeast cells Trevena Reports Favorable TRV045 Topline Safety and Tolerability Data from Proof-of-Concept

Founders often present patents as proof of strength, while investors read them as indicators of risk

in their structural complexity, some members of the RGS family are selective for certain GPCRs, as a proof

Discover how Revvity’s pHSense™ reagents enable real-time GPCR internalization detection A breakthrough challenges in GPCR internalization assays , pHSense™ reagents  combine live-cell, no-wash protocols  with time-resolved fluorescence (TRF) detection , enabling real-time detection of GPCR internalization —without microscopy detecting endogenous GPCRs With pHSense™, scientists can finally track GPCR internalization  in real time—even GPCR Podcast , where he shares the story behind pHSense , including its application in real-time internalization

But over time, he’s learned a skill that transformed his career: pacing. Some weeks, the best use of your time is reading, thinking, and slowing down. Other times, you’ll need to sprint. Knowing the difference is survival.

many biotech startups drift into trouble without ever making a single decision that looks wrong at the time By the time leadership senses friction, the underlying causes are already structural. 👉 Biotech startup By the time misalignment becomes visible, it often shows up indirectly. Execution feels heavier. Over time, this sideways motion becomes costly, both financially and organizationally. 👉 By the time Clarity is not a one-time act. It is a repeated discipline.

Watch Episode 176 The first time Michelle ran a cyclic AMP assay, she did it with a single-channel pipette That’s not a story about nostalgia — it’s a snapshot of how GPCR research was built, on technique at a time We were doing assays on ice, pipetting one sample at a time. result, The pace and precision of GPCR research today — from high-throughput ligand screens to real-time Like many, it was a mix of opportunity, timing, and the courage to say yes before everything was figured

it was difficult, because you see all these projects and you think, ah, I don’t know… but then with time —Alessandro Nicoli He discovered that investing time in people pays off. But at the same time, you pass the same struggle before… you shape the new generation too.”  

post-translational modifications (PTMs), contribute to the high affinity binding to the positively charged groove

And every time your Head of Biology spends each night copy-pasting data instead of thinking about the next experiment, your program is bleeding six figures in lost time and wasted salaries. But here’s the reality: even ultra-specialized experts can’t fix systemic dysfunction in their spare time That’s a chaos tax — and you’re paying it in cash, time, and morale.

Fluorescent ligands provide real-time data on receptor activation, ligand binding and downstream signaling Real-time dynamic labeling of A 2B AR. confocal images of HCT116 cells pre-stained with CellTracker Green (lower panels) and labeled in real-time C) Quantification of CELT-327 average fluorescence intensity over time. when prolonged exposure to light in the excitation range causes the emission signal to decrease over time

“The first time I wore my lucky cactus shirt to a major meeting... and it led to a game-changing collaboration Getting accepted into a PhD program during a personally difficult time.

Yet over time, alignment fades, and decisions start to feel disconnected. 👉 Many founders ask why biotech Over time, focus dissolves. 👉 The company becomes busy instead of deliberate. Over time, confidence erodes. Founders who succeed understand that strategy is not a one-time exercise. Over time, this creates drift that no amount of effort can correct. ✅ Strategy is what holds a biotech

Yet discovery teams routinely lose time and misallocate resources because the underlying biology behaves couple, decouple, isomerize, deplete tracers, and shift apparent affinity depending on stoichiometry and time Equilibration time:  Many assays stop before the system reaches equilibrium, especially with slow competitors You may see: Early stopping → potency distortions Different stopping times → incomparable datasets Curve shape → hints about missing equilibrium Real-time binding systems remove the guesswork.

. 👉 BD partners disengage  because timing and readiness seem inconsistent. 👉 Teams hedge their work reactivity, heroic sprints, long silences, last-minute preparation, and shifting assumptions about BD timing But what kills momentum is timing inconsistency . 👉 Without a defined decision cadence: Choices get  of progress. 👉 Weak cadence leads to: sporadic investor updates BD conversations that lack mutual timing

And most of the time, the issue isn’t style. If your pitch doesn’t address timing, portfolio alignment, or internal traction, the audience won’t do it addresses. ✅ Frame the situation in terms of what’s at stake, whether that’s patient outcomes, time It’s about strategic clarity. ✅ When your pitch follows this logic, it respects the listener’s time, It communicates that you know who you’re building for, why now is the right time, and how your solution

Watch Episode 169 What if you could earn a PhD while supporting a family and working full time in drug With a growing family and a full-time position, traditional PhD training just wasn’t an option.

potency errors from premature reads ✅ Methods to detect hidden mechanisms—mixtures, dual effects, or time-dependent catching a train: two passengers have tickets (affinity), but only the one who sprints to the platform on time But that’s timing, not a different kind of corn. That’s not pharmacology. That’s kinetics. activities before they waste resources Classify antagonists by offset rate without waiting on PK data Spot time-dependent

Yet one well-timed question can unlock clarity, accelerate a stalled project, or even spark a collaboration Fear of wasting someone’s time. Science Late in the conversation, JB offers advice that should be printed on the badge of every first-time

The time you’ll save is yours. Terry’s Corner is coming soon. Dr. The time you’ll save is yours. Terry’s Corner is coming soon. Stay curious, The Dr. GPCR Team

Early In Vivo Wins the Race When is the right time to move beyond cell assays? “Target residence time in vivo correlates beautifully with activity. Modern real-time assays can deliver these insights earlier, faster, and cheaper than most teams assume Every misaligned study is not just wasted budget—it’s lost time. Program Kill vs.

Over time, this constant adjustment drains confidence and momentum, even when the science is strong. Over time, founders compensate by explaining more, not realizing that explanation is a symptom, not a The most damaging effect is subtle. ✅ Founders start adapting their message in real time , trying to mid-conversation , which increases confidence and coherence Alignment can be tested quickly , saving time from founders. 👉 They no longer carry the responsibility of making every detail meaningful in real time

through dramatic moves or bold announcements, but through subtle direction-setting that compounds over time By the time results show up, direction has already been set. Timing shapes results. Early decisions set the direction long before outcomes appear. Over time, this friction compounds and erodes confidence, even when the underlying strategy is sound. It is something you design early, one deliberate decision at a time .

staining is a technique that helps differentiate cells and has been used in flow cytometry for a long time This facilitates the study of receptor dynamics, ligand binding and signaling in real time. the advantages enumerated previously, fluorescent ligands provide new capabilities: -            Real time

and early safety in real development contexts Advance with nuanced lessons on allostery, residence time Read how scientific need and timing drove the leap from academia to application.  

kinetics-based drug development have become immensely interested in predicting the drug target residence time Screening drug candidates in terms of their computationally predicted residence times, which is a measure from steered molecular dynamics simulations and the Bell-Evans model to predict the absolute residence times We have predicted the absolute ligand residence times on the timescale of seconds.

Bioluminescence Resonance Energy Transfer (BRET), has revolutionized the study of GPCRs by enabling real-time One of the significant advantages of FRET and BRET-based sensors is their ability to provide real-time discovery efforts by enabling the precise characterization of pharmacological interventions in real time Galés, C., et al., Real-time monitoring of receptor and G-protein interactions in living cells.  Eidne, Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein