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Elizabeth Johnstone were awarded one of the 2022 Diabetes Research Grants at the World Diabetes Day Breakfast Perkins Professor Kevin Pfleger and Dr Elizabeth Johnstone who were awarded one of the 2022 Diabetes Research This $60,000 grant from Diabetes Research WA will assist the Perkins' Molecular Endocrinology and Pharmacology

Hence, it has wide applicability across a spectrum of neuroscience research, ranging from the study of

October 2022 "Single-molecule counting techniques enable a precise determination of the intracellular abundance and stoichiometry of proteins and macromolecular complexes. These details are often challenging to quantitatively assess yet are essential for our understanding of cellular function. Consider G-protein-coupled receptors-an expansive class of transmembrane signaling proteins that participate in many vital physiological functions making them a popular target for drug development. While early evidence for the role of oligomerization in receptor signaling came from ensemble biochemical and biophysical assays, innovations in single-molecule measurements are now driving a paradigm shift in our understanding of its relevance. Here, we review recent developments in single-molecule counting with a focus on photobleaching step counting and the emerging technique of quantitative single-molecule localization microscopy-with a particular emphasis on the potential for these techniques to advance our understanding of the role of oligomerization in G-protein-coupled receptor signaling." Read more at the source #DrGPCR #GPCR #IndustryNews

Exploring Intracellular Targets: Bridging the Gap Between In Vitro and In Vivo GPCR Research - August and a research associate/scientist in in vitro pharmacology. research within the field of Cellular Biochemistry. Discover the research topic ➤ Why Dr. GPCR scientists, translational pharmacologists, biotech drug discovery teams, and decision-makers who

August 2022 Luciferase-based GloSensor™ cAMP assay: Temperature optimization and application to cell-based kinetic studies "G protein-coupled receptors (GPCRs) are an important receptor superfamily and common therapeutic targets. The second messenger cyclic adenosine monophosphate (cAMP) is a key mediator in many GPCR signaling pathways. Monitoring intracellular cAMP levels can help identify orthosteric agonists and antagonists, as well as allosteric modulators. In this regard, luminescence-based biosensors have revolutionized our ability to monitor GPCR signaling kinetics. The GloSensor™ cAMP assay enables real-time monitoring of signaling downstream of many GPCRs. However, it is crucial to optimize assay conditions such as temperature. As well, it has not been reported whether the effects of temperature on biosensor activity are reversible. Here, we describe the temperature sensitivity and reversibility of the GloSensor™ cAMP assay, and which GloSensor™ version is optimal for measuring cytosolic cAMP. We also present a detailed protocol for monitoring cAMP levels in live cells expressing endogenous or exogenous GPCRs. Temperature optimization studies were carried out using HEK293H cells transiently transfected with the adenosine receptor A2a and the GloSensor™ plasmid (pGloSensor-20F or -22F). We found that preincubation and luminescence reading at room temperature were optimal as compared to higher temperatures. As well, the GloSensor-22F biosensor had a superior signal-to-background ratio and the effect of temperature on biosensor activity was reversible. However, thermal instability of the biosensor may pose a problem for in vivo studies. Nevertheless, the GloSensor™ cAMP assay can be applied to analyze signaling by a wide range of GPCRs for drug discovery" Read more at the source #DrGPCR #GPCR #IndustryNews

Whether you’re optimizing screening campaigns or navigating translational challenges, these insights moments that changed everything. 👉 Discover the pHSense™ Reagents + Listen to the podcast ➤ Celtarys Research A new contributor article from our friends at Celtrays Research outlines how dual-labeled fluorescent decision-makers. 🚀 Join Premium Today & Enjoy ➤ 🎓 Full GPCR University + 🔬 200+ expert talks 🗞️ Weekly research networking, AMAs & matchmaking 💡 Support open resources for the global GPCR field 🧠 Designed for researchers

GPCR research requires turning data into insights for drug discovery Hi GPCR Friends, Every week, critical decisions in GPCR research hinge on subtle shifts in data. Upcoming events:  Major GPCR-focused symposiums and translational meetings are on the horizon — shaping Career opportunities:  Leadership-level roles in clinical operations and translational science, alongside GPCR scientists, translational pharmacologists, biotech discovery teams, and decision-makers who need

Celtarys Research expands its assay tools with CELT-419 for D3 receptor studies, and Dr. Terry Kenakin brings translational clarity to complex receptor concepts.   The Kenakin Brief Fluorescent Probe CELT-419 Powers D3R Binding Assays Across Platforms   Celtarys Research Maria Majellaro’s path from lab research to co-founding Celtarys, as told in her recent Dr. Explore this week’s research, tools, and biotech insights in one place.

, This week's breakthroughs are crucial for staying ahead in the rapidly evolving landscape of GPCR research Career opportunities:  Explore a selection of high-level job openings in high-throughput screening, research Catherine’s research, blending GPCR signaling studies with public health data, offers critical insights Competitive Edge:  Stay informed on emerging public health threats and the scientific research aimed Shape the next generation of cellular biochemistry research.

Enhance your GPCR research with deeper assay insights for more effective results. Plus: new research on CaSR, leptin signaling, and inflammation-linked GPCRs — and curated roles in computational Design for translation:  Map sensitivity ranges to tissue contexts so your plate data predicts what happens Become a Premium Member today. ➤ 🎓 Full GPCR University + 🔬 200+ expert talks 🗞️ Weekly research, networking, AMAs & matchmaking 💡 Support open resources for the global GPCR field 🧠 Designed for researchers

November 22, 2021 JUPITER, FL—Scientists at Scripps Research in Florida have created a collection of

high-impact insight into GPCR allostery, crafted for pharmacologists and biotech scientists who need to translate Career opportunities:  Exclusive pharma and academic listings you won’t find on job boards—curated for translational Position your research for maximum visibility.   If you work on GPCRs across translational pharmacology, drug development, or molecular pharmacology, GPCR scientists, translational pharmacologists, biotech drug discovery teams, and decision-makers who

Exploring the Hidden Dynamics: How Kinetics Reveals What Equilibrium Conceals in GPCR Research. members get to rewatch the recording Subscribe to the Kenakin Brief and Join the Live AMA ➤ Celtarys Research GPCR scientists, translational pharmacologists, biotech discovery teams, and decision-makers who need master GPCR science — in one membership. 🎓 Full GPCR University + 🔬 200+ expert talks 🗞️ Weekly research networking, AMAs & matchmaking 💡 Support open resources for the global GPCR field 🧠 Designed for researchers

Why CYP450 allostery can make or break translation from bench to bedside. He argues that early-career researchers often fall into the trap of over-rationalizing too soon—missing GPCR scientists, translational pharmacologists, biotech discovery teams, and decision-makers who need Become a Premium Member today. ➤ 🎓 Full GPCR University + 🔬 200+ expert talks 🗞️ Weekly research, networking, AMAs & matchmaking 💡 Support open resources for the global GPCR field 🧠 Designed for researchers

and kidney fibrosis and pathological angiogenesis, including occurrence of endothelial-to-mesenchymal transition

Fluorescent probes illuminate the mysteries of GPCRs in cutting-edge research. God Molecule"; Novo Nordisk looks to next generation of obesity, diabetes drugs with $550M Replicate research Career opportunities:  Research Assistant; Postdoctoral Associate; Senior Scientist, Data Science—curated roles aligned to GPCR discovery and translational pharmacology. Join Terry's Corner Today ➤ Celtarys Research – Confocal Imaging That Preserves GPCR Function Confocal

Since then, the National Human Genome Research Institute (NHGRI) has supported annual events across the country to celebrate, learn and discuss the latest advances in genomic research. This intersection of genomics and GPCR research sparked a new era of a comprehensive understanding of References National Human Genome Research Institute, U. S. (2002) National Human Genome Research Institute.

However, in epithelial cells, OGR1 promotes epithelial to mesenchymal transition (EMT) and inflammation

November 2022 "Neuropeptides produce robust effects on behavior across species, and recent research has Neuropeptides exhibit distinct characteristics which includes their post-translational processing, release Current research has focused on isolating when and how neuropeptide transmission occurs, as well as the

Career opportunities:  Roles spanning research associates to principal scientists across membrane proteins From Bench Frustrations to Breakthrough Design For decades, GPCR trafficking research relied on overexpression chemistry can shift the entire GPCR toolkit. 👉 Go behind the scenes with Revvity’s R&D team ➤ Celtarys Research Because they give researchers something rare in CNS work: clarity in complexity. start here 🚀 Join Premium Today & Enjoy ➤ 🎓 Full GPCR University + 🔬 200+ expert talks 🗞️ Weekly research

August 2022 "A confluence of factors has renewed interest in the scientific understanding and translational desire for additional approaches to mental health care, incremental progress in basic and clinical research

Kenakin’s newest foundational lesson in Terry's Corner  cuts straight to it:   Why models are vital for translating

Meanwhile, researchers in the building across the way turned off the lights at 6 PM. Hodson made what seemed like a purely tactical decision: choose a research elective to focus on exams That choice led him to immunology research on pigs — and, eventually, to a PhD. For real translational understanding, you need to see  the receptor in context. That partnership eventually produced fluorescent GPCR tools  that allow researchers to visualize GPCR

We have gathered 24 new research papers on GPCRs and updates on industry news related to GPCRs. Kawthar Belkacemi, Philippe Rondard, Jean-Philippe Pin, and Laurent Prézeau, for their research on Heterodimers receptor Astrocytic GPCR signaling in the anterior cingulate cortex modulates decision making in rats Translation-independent ACKR3 Revealed by Single-Molecule Analysis of their Conformational Landscapes Methods & Updates in GPCR Research HIGHLIGHT Principal Scientist, In vitro pharmacology Post-Doctoral Scientist- Molecular Pharmacology (FDE) Research

The Hidden Lever in GPCR Research In GPCR pharmacology, the conversation often centers on ligand properties—affinity By reducing receptor density, researchers can transform full agonists into partial ones—allowing comparative An assay that reveals drug properties earlier in the pipeline translates directly into better prioritization Assay volume control becomes a tool for risk management, translational prediction, and mechanistic insight

Recent research has unveiled the emergence of G-protein-independent pathways, particularly those involving Once activated, ERKs translocate to the nucleus, phosphorylating various transcription factors, including The nuclear pathway typically involves ERK translocating into the nucleus to regulate gene expression By understanding the intricacies of GPCR signaling and utilising advanced assay technologies, researchers Current Chemical Genomics and Translational Medicine, 7, 9. Kenakin, T. (2019).

The guidelines will aid consistency and clarity, as the basic receptor research and drug discovery communities and should benefit from and contribute to the implementation of the guidelines, together improving translation

relied on: Antibodies of inconsistent specificity Fixed tissue sections Indirect signaling readouts Researchers It’s about how translation happens. Experiments fail for reasons that contain information. conversation is part of a three episode series produced in collaboration with our partners at Celtarys Research

Here’s what I bring: Biology-First Strategy I’ve designed GPCR assays, led collaborations across research environments, and translated complex data into action. Firms  – Looking to accelerate, troubleshoot, or validate GPCR assets in their portfolio. ✅ Contract Research

importance of functional selectivity/biased signaling has led to the discovery of biased compounds as both research We discuss the importance of understanding the translatability of cell and animal models to have more